Hello everyone,
Juts a couple of quick questions:
1) how to increase memory usage for artemis? I tried to add java -mx250m -jar in front of my path in target field, but it just won't work. That should work (I took it from Sanger webpage), but there must be a small detail that I'm not doing right.
2) when I load sorted BAM file of RNA-seq reads against my reference genome, for some regions/genes I see some kind of peak i.e. reads seamed to reach a maximum and they are all equal (look at the picture, in red). That's certainly not normal... you guys have any idea what is it about?
3) There are two colors in my alignment in artemis (see picture). Why's that? I have single-end RNA-seq data and my libraries were not strand specific...
4) If I load two or more replicates as separate files, what artemis displays is a mean of my different biological replicates?
Thanks!
Juts a couple of quick questions:
1) how to increase memory usage for artemis? I tried to add java -mx250m -jar in front of my path in target field, but it just won't work. That should work (I took it from Sanger webpage), but there must be a small detail that I'm not doing right.
2) when I load sorted BAM file of RNA-seq reads against my reference genome, for some regions/genes I see some kind of peak i.e. reads seamed to reach a maximum and they are all equal (look at the picture, in red). That's certainly not normal... you guys have any idea what is it about?
3) There are two colors in my alignment in artemis (see picture). Why's that? I have single-end RNA-seq data and my libraries were not strand specific...
4) If I load two or more replicates as separate files, what artemis displays is a mean of my different biological replicates?
Thanks!
Comment