The different between fragment and reads? thanks anyway！
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Fragment is an universal term for short DNA strings. Read is produced by sequencing machines. -
I would say in the case of paired-end reads, the fragment is the entire piece of which the ends have been sequenced, whereas the reads are just the sequenced parts. If that's what you're talking about.
Like cufflinks has "FPKM" fragments per kilobase per million instead of "RPKM" reads per kilobase per million.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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