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  • oiluig87
    Junior Member
    • Nov 2012
    • 3

    Tophat error

    I've got this error when i've trying to map my reads against Hg19 genome using gencode v14 as reference GTF file:


    [2012-11-10 17:51:33] Beginning TopHat run (v2.0.6)
    -----------------------------------------------
    [2012-11-10 17:51:33] Checking for Bowtie
    Bowtie version: 2.0.2.0
    [2012-11-10 17:51:33] Checking for Samtools
    Samtools version: 0.1.16.0
    [2012-11-10 17:51:33] Checking for Bowtie index files
    [2012-11-10 17:51:33] Checking for reference FASTA file
    [2012-11-10 17:51:33] Generating SAM header for /home/me/bin/Indexed_Genome
    /hg19/hg19
    format: fastq
    quality scale: phred33 (default)
    [2012-11-10 17:51:34] Reading known junctions from GTF file
    [2012-11-10 17:52:14] Preparing reads
    left reads: min. length=50, max. length=50, 83657898 kept reads (3515 d
    iscarded)
    [2012-11-10 18:19:37] Creating transcriptome data files..
    [2012-11-10 18:21:43] Building Bowtie index from gencode.v14.annotation.fa
    [2012-11-10 22:46:26] Mapping left_kept_reads to transcriptome gencode.v14.annot
    ation with Bowtie2
    [2012-11-11 07:16:33] Resuming TopHat pipeline with unmapped reads
    [2012-11-11 07:16:33] Mapping left_kept_reads.m2g_um to genome hg19 with Bowtie2

    [FAILED]
    Error running bowtie:

    What can be the cause of it?
    Thanks

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