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  • twotwo
    Member
    • May 2012
    • 40

    PICARD/ReorderSam reference problem

    I got the sam file from the tophat with UCSC reference. Then I ran PICARD/ReorderSam with the reference from UCSC, which downloaded from the tophat website. And the reference folder contained .fa, .dict and .fa.fai. However the ReorderSam always gave me error message.

    Here is my code:
    java -jar /home/software/picard-tools-1.79/ReorderSam.jar I=/mnt/mutation/bamfile/sample1_f.bam O=/mnt/mutation/bamfile/sample1_sorted_reorder.bam R=/home//reference/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa

    Here is the error message:

    Exception in thread "main" net.sf.picard.PicardException: New reference sequence does not contain a matching contig for chr11_gl000202_random

    Any comments on that???
  • miguelmata
    Junior Member
    • Jul 2014
    • 1

    #2
    I also have the same issue, have you been able to resolve it?

    Comment

    • Vandelnokk
      Junior Member
      • Oct 2012
      • 2

      #3
      By default the tool requires an exact match -- to relax that requirement, use ALLOW_INCOMPLETE_DICT_CONCORDANCE=TRUE

      Comment

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