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  • shuang
    Senior Member
    • Jul 2011
    • 100

    #1

    How to generate my own libarary for RepeatMasker

    I want to generate my own library for RepeatMasker.

    I plan to generate a library based on Plant Repeat Databases at Michigan State University (http://plantrepeats.plantbiology.msu.edu/downloads.html). How do I start from there??


    The file content is in fasta, eg

    >ARSiRGRR00500001 gi|29468390|gb|AF497419.1| Arabidopsis suecica clone JS6-3924 5S ribosomal RNA gene, partial sequence
    GACAACTTGGCAGGGATACCTTTTCGGAAAGCCCAAAGAGAGCCCTCGGACGAAAGAAGC
    AGGACAATGAACTTTTCCATTGACTTTTTGTCGACTCCAAATTTTGACCTTTAAGTACTT
    TTTCGGGCATTTTCGTGATTTGTGCTATATTACGGACCCAAAATTACTCTTTCAAGCATT
    GTTTTCGAATATTTTTCGTGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATG
    GCGGGTAGGACCCACGGCGAACGGTTCATTCAAGACTTAAAAAAAGAATATATACGATTG
    CATTGCATATACTAACGGCTGCGATCATACCAGCACTAATGCACCGGATCCCATCAGAAA
    TCCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTAACTAGGATGGGTGACCTCCCGGGAAGT
    CCTCGTGTTGCAGCCCTCTTTTTTTTTTTTT
    >ARSiRGRR00500002 gi|29468389|gb|AF497418.1| Arabidopsis suecica clone JS6-3925 5S ribosomal RNA gene, partial sequence
    GCCAAACTTGGCATGTGATACCTTTTCGGAAAGCCCAAAGACAGCCCTCCGACGAAATAA
    GCAGGACAATGGAATTTTCCATTGACTTTTTGTCGACCCCAAATTTTGACCTTTAAGTAC
    TTTTTCGGGCATTTTCGTGATTTGGGCTATATTACGGACCCAAAATTACTTGTTCAAGCA
    TTGTTTTCGAATTTTTTCATGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACAT
    AGCGGGTGGGACCCACGGCGAATGGTTCATCAAGTCTTCAAAAAAGAATATATACGATTG
    CATTGCATATACTAACGGATGCGATCATACCAGCACTAATGCACCGGATCCCATCAGAAC
    TCCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTACTAGGATGGGTGACCTCCCGGGAAGTC
    CTCGTGTTGCATCCCTCTTTTATGTTTAACCTTTT
  • SES
    Senior Member
    • Mar 2010
    • 275

    #2
    The "-lib" option is what you want. The argument is just a Fasta file.

    Comment

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