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  • OTU representatives analysis in MEGAN

    Hi,


    I have read the MEGAN manual, and I came upon the following:

    "By default, the program requires at least 5 reads to hit a taxon, before the taxon is deemed present. All reads that are initially assigned to a taxon that is not deemed present are pushed up the taxonomy until a node is reached that has enough reads."

    Now as I am working with representative sequences from OTUs, I want to change this setting. Does anyone know how to change this, so that only 1 read is required to hit a taxon, before the taxon is deemed present?

    For the record I am working with pyrosequencing 16S reads.


    Any advice will be greatly appreciated!

    Kind Regards,

    Sam (graduate student from Belgium)

  • #2
    My apologies, I found it a bit further in the manual

    case solved

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