hello everyone!
I got two samples bisufite sequencing data(each about 10G from illumina platform).after rowdata QC and alignment as well as methylation calling by Bismark,now i have methylation level in each sample's C (CpG,CHH,CHG region) and wanna perform downstream analysis such as DMR.
here are my question:
1,how to identify DMRs between two sample without replicated?
2, any tools can do this kind of analysis?
3,Is there any wrong within my analysis workflow?(ps:i am a green hand)
I got two samples bisufite sequencing data(each about 10G from illumina platform).after rowdata QC and alignment as well as methylation calling by Bismark,now i have methylation level in each sample's C (CpG,CHH,CHG region) and wanna perform downstream analysis such as DMR.
here are my question:
1,how to identify DMRs between two sample without replicated?
2, any tools can do this kind of analysis?
3,Is there any wrong within my analysis workflow?(ps:i am a green hand)
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