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  • tahamasoodi
    Success
    • May 2012
    • 130

    Control Freec error

    Hi,

    I'm getting the following error while running control freec:

    Unable to open fasta file for chr Vې���,�7+�+o�����i���C-]HϧV@A���Z�r�A��h!�O�'XAX���'�������<M�٤���*F���|FX{�?�AI������h���a��[���x2�NM���G��3�cR��A�������Ҋ�� in folder /home/taha/Softwares/Human-Genome/hg19_chrs/humangenome19

    Please note, /home/taha/Softwares/Human-Genome/hg19_chrs/humangenome19 should be a folder, not a file!


    The input file i use is:

    [general]
    chrLenFile = hs19_1C.len
    window = 3000
    step = 1000
    ploidy = 2
    #breakPointThreshold = -.001
    intercept=1
    minMappabilityPerWindow = 0.7
    outputDir = /media/Disk-2/Freec
    sex=XY
    breakPointType=4
    #degree=3
    #coefficientOfVariation = 0.05
    gemMappabilityFile = /home/taha/Softwares/Gem/hg19/out76_hg19.gem
    chrFiles = /home/taha/Softwares/Human-Genome/hg19_chrs/humangenome19/

    [sample]

    mateCopyNumberFile = /home/taha/Softwares/samtools-0.1.18/505C.sorted.bam
    inputFormat = bam
    mateOrientation = FR

    [control]

    mateCopyNumberFile = /home/taha/Softwares/samtools-0.1.18/505N.sorted.bam
    inputFormat = bam
    mateOrientation = FR

    [BAF]

    SNPfile = snp132.txt
    minimalCoveragePerPosition = 5

    [Target]

    captureRegions = TargetSeq_exome_target_regions_hg19.bed

    Can anybody help?
    Thanks,

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