building consensus with multiple samples

[mht]

Hi,

I have 4 samples with Illumina reads which I would like to build a consensus out of. The 4 samples are of the same species and there is a reference for it. I am looking into using Velvet Columbus, but I was wondering, is there a problem with pooling my reads together and proceeding with one assembly?

My aim is to call SNPs using this consensus.

Much thanks.

[Apexy]

Going by an educated guess, you should pool them together if all are from the same library.

[mht]

Yes, they're all Illumina reads. I was guessing it would be alright too if they're the same species, I have two parents and two progeny. I was just wondering if the presence of SNVs will affect the de novo assembly process.