Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Trudy
    Member
    • Feb 2011
    • 16

    #1

    RABT assembly output

    Hello!

    I've a question about the output of cufflinks with RABT assembly.
    In the isoforms.fpkm_tracking file I have 3 different situations:

    1) ENST00000492842 - - ENSG00000240361 if the software identified transcripts that are included in the gtf reference..isn't it?

    2) CUFF.5.1 - - CUFF.5 if the software identified transcripts that are completely new compared to reference..isn't it?

    and finally
    3) ENST00000428771 - - CUFF.9
    ENST00000304952 - - CUFF.9
    ENST00000481869 - - CUFF.9
    ENST00000484667 - - CUFF.9
    This is the question: if cufflinks doesn't identify new transcripts for this locus (because the 4 related transcripts are known) why the locus (CUFF.9) is not known and the same of the reference? These transcripts have the same coordinate of the reference.

    So.....How can I discriminate between novel transcripts (CUFF.5.1 - - CUFF.5) and the new possible isoforms of a known gene?

    Thanks

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...