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  • kenphi
    Junior Member
    • Nov 2009
    • 2

    cufflinks: Intron not within scaffold

    Hi

    I am using cufflinks 1.3.0 in --GTF-guide (-g) mode to do a guided de novo assembly. I get an extremely large number of "Warning: intron not within scaffold [(num, num), num]" warnings, e.g.:

    Code:
    Warning: intron not within scaffold ([6006916-6008030], 0)
    Warning: intron not within scaffold ([6006917-6008031], -860968736)
    Does anyone know what these warnings mean?
    Thanks for your help!

    Philip

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