I've seen 3'-5' coverage plots used to diagnose RNA degradation for RNA-Seq experiments. Before I begin writing my own I was just wondering if anyone knows of an existing script that can produce them (from a BAM and a GTF for instance)?
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Nice, thanks!
I was also interested in some easy way to get what the geneBody_coverage.py module is plotting.Comment
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Hi, have you tried ngs.plot? It is a very powerful and flexible tool to plot NGS samples. In addition to a global plot for the entire genome, you can create any combination of bam files and gene lists. You can plot RNA-seq or ChIP-seq which are implemented as two different modes.
As for your question, click this example .Last edited by asiangg; 06-19-2013, 09:42 AM.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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