Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • aafc
    Member
    • Dec 2012
    • 10

    #1

    How do you specifiy specific replicates in DESeq?

    I have three replicates that I made a count table for, with 6 conditions each. I am wondering how I can tell DESeq to only compare data between two replicates as opposed to accounting for all three.

    Naively I can just filter out the correct columns in the count table, but I am thinking there has to be a one-liner in DESeq that would do this for me.

    Thanks.
  • aafc
    Member
    • Dec 2012
    • 10

    #2
    I think i figured it out:

    Look at the vingette for DESeq and see how they filtered the replicates for a specfic property in the line:

    > pairedSamples = pasillaDesign$libType == "paired-end"

    So, all you would have to do is make a new array in your design table (called pasillaDesign in the vignette) that has binary values (I used 0 for "this replicate is not in the subset of replicates I want", and 1 for "this replicate is in the subset of replicates I want").

    Suppose this aforementioned array was called subset, then all you do is:

    > pairedSamples = pasillaDesign$subset == "1"

    Comment

    Latest Articles

    Collapse

    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    56 views
    0 reactions
    Last Post kinpict.com  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    Last Post SEQadmin2  
    Working...