Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • ersgupta
    Member
    • Jun 2011
    • 26

    #1

    Error while running ContEst

    I am trying to run ContEst(http://www.broadinstitute.org/cancer/cga/contest)

    Below is the command and options I used:

    java -Xmx1200m -jar ContEst.jar \
    -T Contamination \
    -I test.bam \
    -R hg19_GATK_Ref.fa \
    -L target_bed_file.bed \
    -Bop,vcf hg19_population_stratified_af_hapmap_3.3.with.chr.vcf \
    -B:genotypes,vcf test.vcf \
    -BTI genotypes \
    -o test.vcf.contest.out.txt

    Here is the error report:

    INFO 17:18:47,638 TraversalEngine - chr15:65621857 1.48e+06 5.2 m 3.5 m 72.4% 7.2 m 119.5 s
    ##### ERROR ------------------------------------------------------------------------------------------
    ##### ERROR stack trace
    java.lang.ArrayIndexOutOfBoundsException: 0
    at org.broadinstitute.cga.tools.gatk.walkers.cancer.contamination.ContaminationWalker.map(Unknown Source)
    at org.broadinstitute.cga.tools.gatk.walkers.cancer.contamination.ContaminationWalker.map(Unknown Source)
    at org.broadinstitute.sting.gatk.traversals.TraverseLoci.traverse(TraverseLoci.java:78)
    at org.broadinstitute.sting.gatk.traversals.TraverseLoci.traverse(TraverseLoci.java:19)
    at org.broadinstitute.sting.gatk.executive.LinearMicroScheduler.execute(LinearMicroScheduler.java:62)
    at org.broadinstitute.sting.gatk.GenomeAnalysisEngine.execute(GenomeAnalysisEngine.java:234)
    at org.broadinstitute.sting.gatk.CommandLineExecutable.execute(CommandLineExecutable.java:113)
    at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:221)
    at org.broadinstitute.sting.gatk.CommandLineGATK.main(CommandLineGATK.java:87)
    ##### ERROR ------------------------------------------------------------------------------------------

    It seems to be some issue with chr15 because I tried it with 3 different bams files and the reference used to generate those bams is also same(same as the one I used with ContEst).

    I am unable to find any such issue reported by anyone else.

    Anybody else having this issue or knows a way around ??
    Last edited by ersgupta; 12-19-2012, 07:30 AM.
  • venkateshkp
    Junior Member
    • Aug 2013
    • 1

    #2
    Even I had the same error while running ContEst exactly at chr14-15.
    Try it out with smaller bam files it works!

    Anybody solved this error?

    Comment

    Latest Articles

    Collapse

    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    56 views
    0 reactions
    Last Post kinpict.com  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    Last Post SEQadmin2  
    Working...