Hi! everyone! Now i have 2 sRNA high-throughput seqencing library that include 1000,0000 clean reads, i want to search potential siRNA according to criterion that the two perfectly complementary sRNAs with 2 nt overhangs at the 3-end were considered to be siRNA. Tags from clean reads were aligned with each other by blast+,but blast is very slowly! Can anyone tell me some better pipeline or scripts such as perl ? i would be be deeply grateful!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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