Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • NBlackburn
    Junior Member
    • Mar 2012
    • 3

    #1

    Errors loading particular vcf files into IGV but not others

    Hi,

    (would've posted this in the google group but it's not letting me for some reason, so I thought I'd try SEQanswers instead)

    IGV version: 2.2

    What I did:
    - File -> Load from file -> indels.vcf

    What I expected to happen:
    Loading of indel variations into track of IGV browser

    What actually happened:
    Error loading /filepath/Indels.vcf: Line: chr1 821351 . TGCTGTGGTT----- TAGGGCTGATATGGA 357 PASS DP=55 GT:GQ 0/1:357

    Thought it might be a problem with the index for this vcf file, so reindexed it with igvtools and the following error came up whilst running igvtools:
    Error: Line: chr1 821351 . TGCTGTGGTT----- TAGGGCTGATATGGA 357 PASS DP=55 GT:GQ 0/1:357

    This is an Illumina generated file, I have other vcf files from other individual vcf files from the same batch that load into IGV fine (and some that have similar errors including: Error: The provided VCF file is malformed at approximately line number 110: Unparsable vcf record with allele TGCTGTGGTT----- when using igvtools to index).

    This file and others have loaded fine into previous versions of IGV. I just haven't accessed it for a while.

    Any thoughts on what's going wrong?

    Cheers,

    Nick
    Nicholas Blackburn | PhD Candidate
    Tasmanian Inherited Cancer Centre
    Menzies Research Institute Tasmania
    Hobart TAS 7000 | www.menzies.utas.edu.au
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Maybe it is an old VCF format? IGV 2.2 read VCF version 4.0.

    Comment

    • NBlackburn
      Junior Member
      • Mar 2012
      • 3

      #3
      Nice thinking TiborNagy, but they're all VCF versions 4.1.
      Nicholas Blackburn | PhD Candidate
      Tasmanian Inherited Cancer Centre
      Menzies Research Institute Tasmania
      Hobart TAS 7000 | www.menzies.utas.edu.au

      Comment

      Latest Articles

      Collapse

      • kinpict.com
        Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by kinpict.com
        The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
        Today, 06:37 PM
      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 11-12-2026, 07:59 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      35 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      2 responses
      56 views
      0 reactions
      Last Post kinpict.com  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      175 views
      0 reactions
      Last Post SEQadmin2  
      Working...