Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • cliff
    Member
    • Oct 2009
    • 41

    #1

    Question about MAQ Easyrun

    Hey, all

    I am new to MAQ and am frustrated about the easyrun function. I tried the following command on a single-end fastq file:

    maq.pl easyrun -d /..../usr chr1.fa s_1_1_sequence.fastq

    It took about 5 hours to finish this run and the below is the report:
    -- == statmap report ==
    -- # single end (SE) reads: 18980757 (because I just used read)
    -- # mapped SE reads: 1772399 (/ 18980757 = 9.33%)
    -- # paired end (PE) reads: 0
    -- # mapped PE reads: 0 (/ 0 = NA%)
    -- # reads that are mapped in pairs: 0 (/ 0 = NA%)
    -- # Q>=30 reads that are moved to meet mate-pair requirement: 0 (/ 0 = NA%)
    -- # Q<30 reads that are moved to meet mate-pair requirement: 0 (NA%)

    Since our expriment design is paired-end, I tried again for two fastq files.

    maq.pl easyrun -d /..../usr chr1.fa -p s_1_1_sequence.fastq s_1_2_sequence.fastq

    I specified "-p" here because there are two input read sequence files. But this time, the run failed.

    I can't figure it out why this can't work. Can anybody here help me on this?

    All your help will be greatly appreciated!

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...