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  • cliff
    Member
    • Oct 2009
    • 41

    #1

    Question about MAQ Easyrun

    Hey, all

    I am new to MAQ and am frustrated about the easyrun function. I tried the following command on a single-end fastq file:

    maq.pl easyrun -d /..../usr chr1.fa s_1_1_sequence.fastq

    It took about 5 hours to finish this run and the below is the report:
    -- == statmap report ==
    -- # single end (SE) reads: 18980757 (because I just used read)
    -- # mapped SE reads: 1772399 (/ 18980757 = 9.33%)
    -- # paired end (PE) reads: 0
    -- # mapped PE reads: 0 (/ 0 = NA%)
    -- # reads that are mapped in pairs: 0 (/ 0 = NA%)
    -- # Q>=30 reads that are moved to meet mate-pair requirement: 0 (/ 0 = NA%)
    -- # Q<30 reads that are moved to meet mate-pair requirement: 0 (NA%)

    Since our expriment design is paired-end, I tried again for two fastq files.

    maq.pl easyrun -d /..../usr chr1.fa -p s_1_1_sequence.fastq s_1_2_sequence.fastq

    I specified "-p" here because there are two input read sequence files. But this time, the run failed.

    I can't figure it out why this can't work. Can anybody here help me on this?

    All your help will be greatly appreciated!

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