what is the difference between chimeric hits and multiple hits? Does chimeric hits affects the SNP detection in further downstream analysis? If the answer is positive then can any1 suggest me the software for removing the chiemeric hits???
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Multiple hits: a read that maps to more than one locationOriginally posted by Maulik23 View Post
Chimeric hits: a read that maps partially at two locations or more (the "SplitRead" method)
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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