Fasta to Ace conversion

[kmcarr]

Farhat,

I don't think it is possible to do what you are asking. FASTA files only contain ID/definition line(s) followed by sequence line(s). You may also have an accompanying quality score file. An ACE file contains much more information than this. For each contig (an ACE file may include more than one contig) it will contain the gapped sequence and quality scores, the gapped sequences of the constituent reads as well as offset information indicating where each of the constituent reads is located on the contig (reference). This information does not exist in the FASTA files so it would be impossible to construct a meaningful ACE file.

[Torst]

Is there a program to convert a Fasta file to an Ace assembly file?

Can you be a bit more precise on what you require?
A FASTA file is just a bunch of sequences with an ID and a description.
What form do you want the ACE file to take?

[Farhat]

I am looking for it for Eagleview as well.

-Farhat

[bioinfosm]

I am looking for the exact same tool ... fasta to ace, but have not succeeded yet.
If it can use quality values, even better...

the ace file can then be used by eagleView to visualize reads on reference

[Farhat]

Is there a program to convert a Fasta file to an Ace assembly file? While googling I came across references to fasta2ace.pl but no program itself.

Thanks.