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  • cascoamarillo
    Senior Member
    • Oct 2010
    • 164

    #1

    GenSequer output

    Hi,
    I'm trying to pull out all the putative introns given a reference sequence and a set of EST. I find out GenSeqer (http://brendelgroup.org/bioinformatics2go/GeneSeqer.php) as a powerful tool for this approach which also takes into account different splice models; great!. But I don't know what to do with the output file; which is like a kind of genbank output and probably can be loaded into gene browsers. It is like this:

    Code:
    EST sequence      3 +strand   263 n (File: gi-9785390+)
    
         1  GAGAGACCTC TGGCTCTGTA TCGCTCGCTG CTCTTCCTCC CACAGATCGA AAACCATGAA
        61  TCCTGAGTAC GACTATCTTT TCAAGCTCCT GCTTATCGGG GATTCTGGCG TAGGCAAGTC
       121  TTGTCTTCTT TTGAGATTCT CTGATGATTC TTATGTAGAA AGTTACATTA GCACTATTGG
       181  AGTCGATTTT AAAATTAGGA CTGTGGAACA AGATGGCAAA ACAATTAAGC TCCAAATTTG
       241  GGACACTGCT GGTCAAGAAC GGT
    
    Predicted gene structure (within gDNA segment 52894 to 50989):
    
     Exon  1  52231  52163 (  69 n);  cDNA      1     69 (  69 n); score: 0.986
      Intron  1  52162  52056 ( 107 n); Pd: 0.902 (s: 1.00), Pa: 0.886 (s: 1.00)
     Exon  2  52055  51983 (  73 n);  cDNA     70    142 (  73 n); score: 1.000
      Intron  2  51982  51897 (  86 n); Pd: 0.978 (s: 1.00), Pa: 0.990 (s: 1.00)
     Exon  3  51896  51849 (  48 n);  cDNA    143    190 (  48 n); score: 1.000
      Intron  3  51848  51759 (  90 n); Pd: 0.637 (s: 1.00), Pa: 0.963 (s: 1.00)
     Exon  4  51758  51711 (  48 n);  cDNA    191    238 (  48 n); score: 1.000
      Intron  4  51710  51624 (  87 n); Pd: 0.977 (s: 1.00), Pa: 0.986 (s:    0)
     Exon  5  51623  51599 (  25 n);  cDNA    239    263 (  25 n); score: 1.000
    
    MATCH	SQ;L89959-	gi-9785390+	0.993	263	1.000	C
    PGS_SQ;L89959-_gi-9785390+	(52231  52163,52055  51983,51896  51849,51758  51711,51623  51599)
    
    Alignment (genomic DNA sequence = upper lines):
    
    GAGAGATCTC TGGCTCTGTA TCGCTCGCTG CTCTTCCTCC CACAGATCGA AAACCATGAA    52172
    |||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| 
    GAGAGACCTC TGGCTCTGTA TCGCTCGCTG CTCTTCCTCC CACAGATCGA AAACCATGAA       60
    
    
    TCCTGAGTAG TAAGTTCCTT TCTCCATCGA CACATACTTG GGTCGAAATT ACCTCTGTTA    52112
    |||||||||                                                         
    TCCTGAGTA. .......... .......... .......... .......... ..........       69
    In my case, I just want to take the intron regions (like fasta file) from the whole genome and make an Introme dataset; though I'm not sure how to do it with this format. Any Idea? Thanks
    Last edited by cascoamarillo; 01-30-2013, 03:52 PM.

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