cummeRbund analysis - genes & CDS

[SHeaph]

Hi All,

I produced the following table from a large data set using cufflinks-cuffmerge-cuffdiff. I produced a heatmap with cummeRbund based on the most significant differentially expressed genes at alpha =0.001. I also produced an expressionBarplot for CDS (alpha =0.001). There were more genes in the barplot than identified in the heatmap.

Can someone please advise what the differences is between both groups?

genes 65536
isoforms 93744
TSS 74511
CDS 29258
promoters 65536
splicing 74511
relCDS 18751


Many thanks in advance!

[sdas125]

genes & CDS

CDS signify coding sequences.
Entire mRNA transcript do not translate into protein. mRNA transcript contains both 5'UTR and 3" UTR, which has versatile functions. Only a part of transcript translate into protein.
CDS is the part of gene sequence (mRNA sequence) that translate to protein. Generally CDS is a small fraction of entire gene sequence (mRNA sequence).