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  • mdb@wistar
    Junior Member
    • Jan 2013
    • 9

    #1

    bowtie2-inspect

    I want to use tophat2 with reference gtf file from UCSC genome browser for which I am supposed to check that the values in the first column of the provided GTF must match the name of the reference sequence in the Bowtie index.
    But to check this I tried the Bowtie2-inspect command:

    bowtie2-inspect -s hg19index.fa as well as bowtie2-inspect -i hg19index.fa

    I get this error:
    "Could not locate a Bowtie index corresponding to basename "hg19index.fa""

    But the index file worked fine with Bowtie2 as well as tophat2.

    Am I making a mistake here, pl clarify,
    thanks
  • westerman
    Rick Westerman
    • Jun 2008
    • 1104

    #2
    Should work. Can you post an 'ls -l' of your directory please.

    Comment

    • mdb@wistar
      Junior Member
      • Jan 2013
      • 9

      #3
      Thanks, i was able to solve the problem by renaming hg19.fa to hg19index.fa

      Comment

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