I've got some list of differentially expressed genes.
Then, as a next step, I want to do blast.
Before doing blast, I want to make a new fasta file.
Regarding the genes that are identified as differentially expressed,
I would like to extract the position of those genes from annotation.gtf,
and, based on this position, extract the sequence from reference.fasta.
Then, with this new fasta file, I want to do blast.
Would you recommend some way to do this? Is there a software which has this function other than galaxy or gffread?
Thank you in advance.
Then, as a next step, I want to do blast.
Before doing blast, I want to make a new fasta file.
Regarding the genes that are identified as differentially expressed,
I would like to extract the position of those genes from annotation.gtf,
and, based on this position, extract the sequence from reference.fasta.
Then, with this new fasta file, I want to do blast.
Would you recommend some way to do this? Is there a software which has this function other than galaxy or gffread?
Thank you in advance.