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  • bgarchow
    Junior Member
    • Feb 2013
    • 1

    #1

    What to do with a *.bowtie CLIP-Seq dataset from GEO?

    Hi all,

    Dumb biologist here so please be gentle. I have a *.bowtie CLIP-Seq dataset downloaded from the NCBI GEO website and I have no idea what to do with it.

    The experiment was immunoprecipitation of an RNA binding protein followed by RNA-Seq to sequence the RNA species bound (crosslinked) to it.

    I would really appreciate it if someone could outline the steps I would need to take to extract meaningful data (i.e. a list of the bound RNA species and confidence values).

    Thanks!

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