Mosaik assembly error

[amcrisan]

I get the following error when running Mosaik assembler... it starts of just fine, however after finishing up the chromosome 1 I get the following error:

- saving alignments from 10:
ERROR: An attempt was made to get reads from an alignment archive that hasn't been opened yet.

I supply it a sorted aligned file, and would like it output in the gigbayes format so that I can use the GigaBayes program for SNP discovery. I tried making the -f file be both ace and gig and they both provide the same error.

Any suggestions?

Thanks.

[yosemite]

Are you using colorspace (SOLiD) data?

[amcrisan]

I am using Illumina..so no not colour space.

[jerolar]

Hello,

I'm new with the Mosaik package and I got the same error. Everything is fine until MosaikAssembler. I work with 454 reads and I want to assemble them on a set of reference sequences, it's a transcriptome sequencing project. Maybe it's because I have more than one reference sequences, but I see nothing special in the doc how to deal this case of figure.

[mack]

i'm on the same situation as jerolar. anyone emailed the author?

[jerolar]

The bug has been reported to the authors but up to now, there are no reply to this problem. We must check on the mosaik's bug tracking website and report here any useful answer. This is the ID #10:

http://code.google.com/p/mosaik-aligner/issues/list

[dsenalik]

I just hit the same bug, but the new version 1.0.1367 released Nov 19 appears to have
resolved this error, it is working fine for me. I added this same info in the bug tracking website.