I am new to the next-gen sequencing world (and the human genetics world for that matter), and I am trying to analyze a resequencing data set with some older data.
First, I want to know the number of variants in each population (African American, European American, etc).
Second, I want to know how many and which variants are shared amongst the populations.
Third, I want to know which variants (SNPs), if any, are novel.
I am familiar with command-line programs, but I am so new to this field that I don't even know which programs to use.
I have all my data in .vcf file formats. I have been able to use vcftools to extract all the data for the gene of interest. I tried the --diff command in vcftools, but that didn't give me what I want. This seems like a straight-forward issue but I don't know who to ask.
Thanks for any help.
While I am here, I could also use some recommendations on getting up-to-speed on the topics of LD, haplotypes, and other population statistics. I need a bioinformatics/genetics for dummies.
Thanks!
EDIT:
Just want to say that I have tinkered around with PLINK, vcftools, R, and Haploview.
First, I want to know the number of variants in each population (African American, European American, etc).
Second, I want to know how many and which variants are shared amongst the populations.
Third, I want to know which variants (SNPs), if any, are novel.
I am familiar with command-line programs, but I am so new to this field that I don't even know which programs to use.
I have all my data in .vcf file formats. I have been able to use vcftools to extract all the data for the gene of interest. I tried the --diff command in vcftools, but that didn't give me what I want. This seems like a straight-forward issue but I don't know who to ask.
Thanks for any help.
While I am here, I could also use some recommendations on getting up-to-speed on the topics of LD, haplotypes, and other population statistics. I need a bioinformatics/genetics for dummies.
Thanks!
EDIT:
Just want to say that I have tinkered around with PLINK, vcftools, R, and Haploview.
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