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  • soonerSEQ
    Junior Member
    • Nov 2011
    • 3

    #1

    Comparing SNPs amongst populations

    I am new to the next-gen sequencing world (and the human genetics world for that matter), and I am trying to analyze a resequencing data set with some older data.

    First, I want to know the number of variants in each population (African American, European American, etc).

    Second, I want to know how many and which variants are shared amongst the populations.

    Third, I want to know which variants (SNPs), if any, are novel.

    I am familiar with command-line programs, but I am so new to this field that I don't even know which programs to use.

    I have all my data in .vcf file formats. I have been able to use vcftools to extract all the data for the gene of interest. I tried the --diff command in vcftools, but that didn't give me what I want. This seems like a straight-forward issue but I don't know who to ask.

    Thanks for any help.

    While I am here, I could also use some recommendations on getting up-to-speed on the topics of LD, haplotypes, and other population statistics. I need a bioinformatics/genetics for dummies.

    Thanks!

    EDIT:
    Just want to say that I have tinkered around with PLINK, vcftools, R, and Haploview.
  • JackieBadger
    Senior Member
    • Mar 2009
    • 385

    #2
    GATK and samtools are a good place to start

    Comment

    • soonerSEQ
      Junior Member
      • Nov 2011
      • 3

      #3
      Originally posted by JackieBadger View Post
      GATK and samtools are a good place to start
      Thanks - I will add those to my list!

      Edit:

      I was able to print the list of SNPs by using PLINK --write-snplist command. I then compared this list with my other populations to determine which were shared and which weren't (by using a crude method in excel).

      Is there not a more scientific way to do this? Is this even a correct method?
      Last edited by soonerSEQ; 03-01-2013, 09:07 AM. Reason: update

      Comment

      • soonerSEQ
        Junior Member
        • Nov 2011
        • 3

        #4
        Could anyone else weight in here? I could really use some guidance.

        Thanks!

        Comment

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