Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • aurimas
    Junior Member
    • Mar 2013
    • 1

    #1

    Converting contig coordinates to genomic coordinates

    I apologize if this has been answered somewhere else.

    I'm trying to do some analysis on a dataset at http://www.ncbi.nlm.nih.gov/geo/quer...acc=GSM1023125 published as a BED file.

    Unfortunately, the "BED" file is in a bit of a weird format, which looks like semi-processed Bowtie output:

    HTML Code:
    gi|149288852|ref|NC_000067.5|NC_000067	176269827	176269871	HWI-ST363:283:C0ND8ACXX:8:1101:2034:2233	255	-
    gi|149288852|ref|NC_000067.5|NC_000067	154993040	154993084	HWI-ST363:283:C0ND8ACXX:8:1101:3639:2182	255	+
    gi|149338249|ref|NC_000068.6|NC_000068	155564443	155564487	HWI-ST363:283:C0ND8ACXX:8:1101:3703:2191	255	-
    gi|149354223|ref|NC_000070.5|NC_000070	42195036	42195080	HWI-ST363:283:C0ND8ACXX:8:1101:5142:2130	255	-
    gi|149292731|ref|NC_000078.5|NC_000078	79949131	79949175	HWI-ST363:283:C0ND8ACXX:8:1101:5103:2145	255	+
    The coordinates are given as contigs (plus gi numbers, which i can easily strip off) instead of genomic coordinates. I've read around and UCSC genome browser used to support contigs in BED files, but they no longer do. There are also several references to the liftUp tool that converts contigs to genome coordinates, but I can no longer locate any place to download liftUp or its source code. So before I venture into re-writing liftUp, I was wondering if there is another tool that I could use (or maybe a place to download liftUp).

    Any pointers would be greatly appreciated.

Latest Articles

Collapse

  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    07-31-2026, 11:01 AM
  • SEQadmin2
    Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
    by SEQadmin2


    Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

    The systematic characterization of the human proteome has
    ...
    07-20-2026, 11:48 AM
  • SEQadmin2
    Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
    by SEQadmin2



    Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
    ...
    07-09-2026, 11:10 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 10:13 AM
0 responses
13 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-31-2026, 02:55 AM
0 responses
26 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-24-2026, 12:17 PM
0 responses
20 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-23-2026, 11:41 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Working...