read different lengths in fastq
Hi elena,
My experience with Illumina sequencing is that all the reads should be the same length.
Have the reads been trimmed to remove low quality regions or adaptor sequences?
Best wishes,
Maria
-
Hello,
I do not think read length is the problem except the reads incorporate many uncalled and wrong bases. Did you perform read thinning or trimming ? By false positive, do you mean that the reads align elsewhere than expected ordo you mean the reads do not have a hit? What is he range of read lengths? What alignment tool are you using? What are you aligning the reads to.Leave a comment:
-
read different length in fastq
Dear,
I have performed two runs (targeted sequencing) on miseq using 2x150bp protocol. Unusually, I have obtained fastq containing reads of different length, that during alignment step cause misalignment errors (and a lot of false positives). Any idea for the reason? Any idea to fix it?
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Leave a comment: