Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • mastal
    replied
    read different lengths in fastq

    Hi elena,

    My experience with Illumina sequencing is that all the reads should be the same length.

    Have the reads been trimmed to remove low quality regions or adaptor sequences?

    Best wishes,
    Maria

    Leave a comment:


  • Apexy
    replied
    Hello,
    I do not think read length is the problem except the reads incorporate many uncalled and wrong bases. Did you perform read thinning or trimming ? By false positive, do you mean that the reads align elsewhere than expected ordo you mean the reads do not have a hit? What is he range of read lengths? What alignment tool are you using? What are you aligning the reads to.

    Leave a comment:


  • m_elena_bioinfo
    started a topic read different length in fastq

    read different length in fastq

    Dear,
    I have performed two runs (targeted sequencing) on miseq using 2x150bp protocol. Unusually, I have obtained fastq containing reads of different length, that during alignment step cause misalignment errors (and a lot of false positives). Any idea for the reason? Any idea to fix it?

Latest Articles

Collapse

  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM
  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-24-2024, 07:15 AM
0 responses
195 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-23-2024, 10:28 AM
0 responses
218 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-23-2024, 07:35 AM
0 responses
222 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-22-2024, 02:06 PM
0 responses
12 views
0 likes
Last Post seqadmin  
Working...
X