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  • Pinal
    Member
    • Feb 2012
    • 11

    #1

    How to extract unmapped reference sequence from BAM/SAM file.?

    How to extract unmapped region from reference sequence from SAM/BAM file.?
  • SylvainL
    Senior Member
    • Feb 2012
    • 180

    #2
    Hi,

    you can use the function coverageBed included in the bedtools suite and then, (for example using the Rpackage Rsamtools), it is really easy to select the uncovered region of your reference and extract the corresponding sequence...

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    • jfmediatico
      Junior Member
      • Sep 2013
      • 1

      #3
      Hello,

      Does anybody know any other way to extract unmapped regions of the reference sequence from a BAM/SAM/ACE file??
      Biologist and Bioinformatics at Universidad of Barcelona
      Jose F. Sanchez

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