Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Kawaccino
    Member
    • Mar 2013
    • 12

    #1

    FastQC result and cleaning sequence

    Hello,
    I need your help for the cleaning of a sequence. I got a big Fastq file issued from an illumina sequening and even if it was supposed to be cleaned before I got it, when I do a Fastqc test, I obtain in the overrepresented sequences category a long table with about 12 sequences of ribosomal RNA that have no hit as possible sources (I got hits when I blast them on the NCBI Site); they are all similar, it's just some bases of the sequences that vary. And I also got strange results like RNA PCR Primer and of course my adaptators. I got rid of the adaptators by a simple Cutadapt, but I still have the other overrepresented sequences. How can I eliminate them, cause I suppose that cutadapt is not adapted to do that, is it?
    Thank you for all the answer you'll be able to give me.
    K.
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Aside from adapter removal, I don't know that I'd worry about removing ribosomal sequences from the fastq files. Aligning is quick enough to not worry about those and you can filter them out of your GTF file if you want to ignore them downstream.

    Comment

    • Kawaccino
      Member
      • Mar 2013
      • 12

      #3
      Ok, thank you for your answer, I will let them in that case and deal with them later.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
        by SEQadmin2



        CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

        Despite this, “CRISPR helped turn genome editing from a specialized technique into
        ...
        07-31-2026, 11:01 AM
      • SEQadmin2
        Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
        by SEQadmin2


        Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

        The systematic characterization of the human proteome has
        ...
        07-20-2026, 11:48 AM
      • SEQadmin2
        Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
        by SEQadmin2



        Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
        ...
        07-09-2026, 11:10 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Today, 07:41 AM
      0 responses
      9 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 08-03-2026, 10:13 AM
      0 responses
      25 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-31-2026, 02:55 AM
      0 responses
      38 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-24-2026, 12:17 PM
      0 responses
      25 views
      0 reactions
      Last Post SEQadmin2  
      Working...