Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • scondo
    Junior Member
    • Feb 2013
    • 6

    #1

    DWGSIM quality distribution

    Hi,

    i use the following command for illumina 100 PE read simulation. The problem is that all simulated reads had the same quality score. I want to simulate reads with a raw accuracy of about 99,5%. How i must configure the command line to generate reads with a different quality score?

    Command line:
    ./dwgsim -e 0.001-0.01 -E 0.0025-0.0075 -d 175 -s 75 -1 100 -2 100 -C 1 -r 0.00131 -R 0.09166 -X 0.3 -I 1 -c 0 -S 0 -x /home/.../Dwgsim-input/extended_regions_sort_illumina.bed /home/../Dwgsim-input/ucsc_chr1-22XY.fa Read

    Quality score:

    @chr1_22158191_22158224_0_1_0_0_5:0:0_40:0:0_1419/1
    CCCTGGGTGAGGCGGCGCAGCAGGGTCACGGTGTGGAAATGGCCCAGGGCCAGTGGTGTGGGATAGGGGATGGTGGCCTTGCCTGAGCCTACATCGAACC
    +
    !@=;:9887666555444333332222211111111000000000///////////...............------------------,,,,,,,,,,,
    @chr1_172357618_172357549_1_0_0_0_6:0:0_47:0:0_141a/1
    GTGACCTGAGGAAAAAGAGAAATCATAGTTTGCTCACTCCTCCTTTCTGTCTGAACTAATCACTAAAAATGTCTTGTCACTGCCTGCTTTTTAAAAATGA
    +
    !@=;:9887666555444333332222211111111000000000///////////...............------------------,,,,,,,,,,,
    @chr1_153638154_153638289_0_1_0_0_5:0:0_36:0:0_141b/1
    GTGCTCTTAGGCTTTCCACGACTTTGTTCCCCAGGGCAGCAACAGGTTCCACTAATTGACAGAAAATGACCTAATGTTATAATAAATTATTTCTCTAGAC
    +
    !@=;:9887666555444333332222211111111000000000///////////...............------------------,,,,,,,,,,,

    Thanks for your help!

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 07:59 AM
0 responses
13 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
32 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
0 responses
45 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
170 views
0 reactions
Last Post SEQadmin2  
Working...