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  • ernesto
    Junior Member
    • Oct 2008
    • 3
    #1

    MosaikTools

    HI,

    I am trying to use the MosaikTools perl API to access the alignments created by MosaikAligner but when I run the perl sample script that the authors provide (MosaikReaderTest.pl) I got the following error:
    # of bases: 30026
    # of reads: 191

    Archive contains single-end reads.
    Archive contains unsorted reads.
    Archive was generated using 'all' mode.

    Dumping reference sequences:
    - MYBPC3chr11:47309533-47330829, aligned reads: 0, length: 21297, MD5 checksum: AE1B7FB0C634D50776E5F8430035FEFB
    - MYH7chr14:22951787-22974710, aligned reads: 112, length: 22924, MD5 checksum: 4422919F489336A27D982A1FF8432B44
    - TNNI3chr19:60354948-60360912, aligned reads: 0, length: 5965, MD5 checksum: EF21F5BC4EBB9CFB5B81DA598E7AFE9F
    - TNNT2chr1:199594765-199613428, aligned reads: 79, length: 18664, MD5 checksum: F2F5EC154A510F52BF1415394926AFF1

    Dumping read groups:

    ERROR: Tried to read 16777223 bytes, but received only 23838 bytes (FSR03LX09.aligned.dat) [read the partition: LoadNextRead]

    Please, i am a bit lost here, could anyone help me?

    Thanks!.

    Ernesto
    Tags: 454, mosaik
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