Simon and all,
I found another one(see the attached). I used cufflinks to assemble the transcripts and it appears that cufflinks assembled the reads in between these two genes as shown. I went further in ensemble and I realised that this (I mean reads between these two genes) were supported by EST alignments (Sequence: GH304014.1 : HW_FAT_169_A03.ab1 HW cDNA library intermuscular fat Bos taurus cDNA clone 169_A03, mRNA sequence.). This is the first time I'm analysing rna-seq data and I basically don't have a clue as to what's going on.
Is there any inference one could deduce from this?
I found another one(see the attached). I used cufflinks to assemble the transcripts and it appears that cufflinks assembled the reads in between these two genes as shown. I went further in ensemble and I realised that this (I mean reads between these two genes) were supported by EST alignments (Sequence: GH304014.1 : HW_FAT_169_A03.ab1 HW cDNA library intermuscular fat Bos taurus cDNA clone 169_A03, mRNA sequence.). This is the first time I'm analysing rna-seq data and I basically don't have a clue as to what's going on.
Is there any inference one could deduce from this?
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