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Originally posted by HTS
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By default, BFAST outputs all mappings for a read regardless of their quality/score. It is only in the postprocessing step do we use various criteria (specified by the user) to choose the "best" alignment. One of those criteria is to output all alignments, which is what you want. -
Great, thanks a lot! I can't wait to try things outOriginally posted by nilshomer View Post
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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