Hallo Bowtie user,
I have a dateset with all transcripts from human genome. On the other side I have a set of RNA-seq reads (mate pairs/Illumina).
I would like to map these reads against the transcripts. .
I was thinking of using bowtie to accomplish this task, but I am not
very sure about which options or flags I should use. I thought, I have to map my reads using the bowtie option -norc. I have already tried this,
but I am not sure, if It is the rigth one.
Do you think, that these are the wrigth options to run bowtie for this task?
Thanks in advance
Mary
I have a dateset with all transcripts from human genome. On the other side I have a set of RNA-seq reads (mate pairs/Illumina).
I would like to map these reads against the transcripts. .
I was thinking of using bowtie to accomplish this task, but I am not
very sure about which options or flags I should use. I thought, I have to map my reads using the bowtie option -norc. I have already tried this,
but I am not sure, if It is the rigth one.
Do you think, that these are the wrigth options to run bowtie for this task?
Thanks in advance
Mary
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