Hello,
I'm looking to assess differential gene expression using Illumina HiSeq reads. I have a two-factor experimental design (three divergent fish populations each treated at two salinities). I would like to assess differential expression using a two-way model - specifically I want to pinpoint genes for which there are significant interaction effects.
Does anyone know if any of the (many) RNA seq tools available can run a model of this kind (probably a generalized linear model) using these data?
Thanks so much.
I'm looking to assess differential gene expression using Illumina HiSeq reads. I have a two-factor experimental design (three divergent fish populations each treated at two salinities). I would like to assess differential expression using a two-way model - specifically I want to pinpoint genes for which there are significant interaction effects.
Does anyone know if any of the (many) RNA seq tools available can run a model of this kind (probably a generalized linear model) using these data?
Thanks so much.
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