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  • shrujan
    Member
    • Aug 2012
    • 38

    #1

    SRA Toolkit in ubuntu 12.04

    Hi, i have downloaded SRA Toolkit.2.3.2 and tried to run ./fastq-dump in shell or terminal but after executing it is showing error as cannot be executed please resolve this.

    thank you.
  • sklages
    Senior Member
    • May 2008
    • 628

    #2
    Which version of Ubuntu? 32bit? 64bit?

    What is the exact error message you get?

    Here on 12.10 (64bit) using 2.3.2-4 I encounter no problems.

    Comment

    • shrujan
      Member
      • Aug 2012
      • 38

      #3
      Ubuntu 12.04

      Ubuntu 12.04. 64 bit Error is that when i try to execute ./fastq-dump, cannot be executed is coming

      Comment

      • sklages
        Senior Member
        • May 2008
        • 628

        #4
        Just like this (not real):

        bin $ ./fastq-dump
        Cannot be executed

        Please post a correct error message along with your command you executed.

        Is this also true for other programs in bin?
        Last edited by sklages; 04-16-2013, 12:49 PM.

        Comment

        • shrujan
          Member
          • Aug 2012
          • 38

          #5
          erro message

          shrujan@ubuntu:~/Programs/sratoolkit.2.3.2/bin$ ./fastq-dump
          bash: ./fastq-dump: cannot execute binary file

          Comment

          • shrujan
            Member
            • Aug 2012
            • 38

            #6
            yes this type of error is coming for other bin files too.

            Comment

            • sklages
              Senior Member
              • May 2008
              • 628

              #7
              hmm, looks like the sra toolkit which you have downloaded has not been compiled for your system.
              I didn't see any 32bit SRA Toolkit archives, so I assume you have dowloaded the 64bit archive.

              So your Ubuntu system is very likely a 32bit system; what do you get with:
              uname -m

              Comment

              • shrujan
                Member
                • Aug 2012
                • 38

                #8
                i686 comes when i type uname -m

                Comment

                • shrujan
                  Member
                  • Aug 2012
                  • 38

                  #9
                  yes i have downloaded 64 bit archive of SRA Toolkit 2.3.2

                  Comment

                  • shrujan
                    Member
                    • Aug 2012
                    • 38

                    #10
                    Should i have to download 32 bit version ??

                    Comment

                    • sklages
                      Senior Member
                      • May 2008
                      • 628

                      #11
                      So you have a 32bit system (though you wrote to have a 64bit system).
                      You cannot execute 64bit software on 32bit systems. As there is no 32bit SRA toolkit binary available for download you could either try to compile the toolkit from source on your 32bit system (be prepared to run into problems) or simply set up a new system, Ubuntu 64bit.
                      When dealing with big datasets, or with software for big datasets, it is not a bad idea to have a 64bit system for your analyses.

                      addendum: I see that Ubuntu holds a 32bit version of an older version of SRA toolkit, http://packages.ubuntu.com/quantal/science/sra-toolkit. Might be of interest for you.
                      Last edited by sklages; 04-16-2013, 01:31 PM.

                      Comment

                      • shrujan
                        Member
                        • Aug 2012
                        • 38

                        #12
                        Oh, thank you for your guide lines. But i have a doubt regarding reinstalling on a hard disk i.e., i heard that re-installing OS on a hard disk may effect hard disk. Is it true ? Because i have already installed twice. So please clarify this doubt. Thank you

                        Comment

                        • sklages
                          Senior Member
                          • May 2008
                          • 628

                          #13
                          Originally posted by shrujan View Post
                          Oh, thank you for your guide lines. But i have a doubt regarding reinstalling on a hard disk i.e., i heard that re-installing OS on a hard disk may effect hard disk. Is it true ? Because i have already installed twice. So please clarify this doubt. Thank you
                          That's funny, never heard about that :-)

                          Don't worry, this is simply not true. I install twice a year a new version of some linux on my notebook, .. it is still alive ;-)

                          Comment

                          • shrujan
                            Member
                            • Aug 2012
                            • 38

                            #14
                            Thank you. Its a great pleasure sharing views and issues. I will try installing 64 bit version, if it doesn't work i will ping you up. Thanks again

                            Comment

                            • shrujan
                              Member
                              • Aug 2012
                              • 38

                              #15
                              SRA Toolkit error

                              Hi, as you said earlier i have reinstalled with 64 bit ubuntu 12.04 LTS OS and tried to run SRAToolkit.2.3.2 again. But same error message is repeated as
                              shrujan@ubuntu:~/Programs/sratoolkit.2.3.2/bin$ ./fastq-dump
                              bash: ./fastq-dump: No such file or directory

                              Please resolve this.

                              Thank you.

                              Comment

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