I have a fixed step wiggle file which I'm trying to convert to a bigWig file but I'm not getting anywhere. Here are the first few lines of the fixed step and variable step wiggle files.
I've tried converting it to a variable step wiggle file using a C program I found on BioStar here and then ran the resulting variable step wiggle file through Jim Kent's wigToBigWig.pl script with no luck. I keep getting the following error.
I tried removing overlaps(?) using a script a colleague suggested may help but with no luck. I still get the exact same error.
I'd really appreciate any suggestions as to how I can a bigWig out of my fixed step or variable step wiggle file.
Thank you!
I've tried converting it to a variable step wiggle file using a C program I found on BioStar here and then ran the resulting variable step wiggle file through Jim Kent's wigToBigWig.pl script with no luck. I keep getting the following error.
Code:
There's more than one value for Cbre_Contig0 base 3585 (in coordinates that start with 1).
I'd really appreciate any suggestions as to how I can a bigWig out of my fixed step or variable step wiggle file.
Thank you!