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  • wired error profile

    Hi,

    We are using MiSeq to sequence a 350bp PCR fragment (250bp paired-end, multiplex with 100 samples, using dual indexes), since this is PCR prodect, we diluted the library 1:10 to get a better cluster identification, however, the sequencing error looks very wired (while most of the index sequences are well-identified), increased dramatically afther the 90bp on both ends. Any one has met this problem before?

    Any idea would be appreciated
    Attached Files
    Last edited by mingkunli; 04-19-2013, 01:48 AM.

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