Hi,
We are using MiSeq to sequence a 350bp PCR fragment (250bp paired-end, multiplex with 100 samples, using dual indexes), since this is PCR prodect, we diluted the library 1:10 to get a better cluster identification, however, the sequencing error looks very wired (while most of the index sequences are well-identified), increased dramatically afther the 90bp on both ends. Any one has met this problem before?
Any idea would be appreciated
We are using MiSeq to sequence a 350bp PCR fragment (250bp paired-end, multiplex with 100 samples, using dual indexes), since this is PCR prodect, we diluted the library 1:10 to get a better cluster identification, however, the sequencing error looks very wired (while most of the index sequences are well-identified), increased dramatically afther the 90bp on both ends. Any one has met this problem before?
Any idea would be appreciated