Are there any tools available which will look at reference sequence and list out all the possible locations that can be potentially genes? or alternatively is there a tool that can take a sequence as input and predict if it can be a gene?
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I think you are really talking about 'ab initio' gene prediction (see https://en.wikipedia.org/wiki/Gene_prediction) and there are a lot of tools that can do this.
Maybe Maker could be useful to you: http://www.yandell-lab.org/software/maker.html -
kbradnam, Thanks a ton for this real quick reply.. This is exactly what I am looking for. Has anyone run these tools on entire human reference say hg18 or hg19?Originally posted by kbradnam View PostComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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