Hi everybody,
thanks for all the useful posts I found in this blog that helped me for my work.
I am new to bioinformatics and I have a question about multiple genome hits. I work with smallRNA-seq form maize; I would like to calculate the genome coverage taking into account not only reads mapping univocally to the genome but also reads with multiple hits in the genome. I did not find a tool that allow to normalize the coverage by diving, for each read's position, it's coverage to the number of alignments the read has on genome.
I thank everybody that could help me on that issue!
Alis
thanks for all the useful posts I found in this blog that helped me for my work.
I am new to bioinformatics and I have a question about multiple genome hits. I work with smallRNA-seq form maize; I would like to calculate the genome coverage taking into account not only reads mapping univocally to the genome but also reads with multiple hits in the genome. I did not find a tool that allow to normalize the coverage by diving, for each read's position, it's coverage to the number of alignments the read has on genome.
I thank everybody that could help me on that issue!
Alis
