Iontorrent denovo and reference assembler tools

[pari_89]

Hi everyone, I am doing my master's thesis on evaluation of de-novo and reference guided assemblies.

I am using iontorrent fastq and sff files.

I have already got ray and mira in my list.

The other tools like velvet gives extremely short reads. Can you suggest with a series of other tools please that will provide a good assembly. SOApdenovo and ABYss are for shorter reads.

Could someone help me with the reference assemblies as well please?

Thank you.

[maubp]

Try the Roche 454 'Newbler' gsAssember too, I've heard it works well with IonTorrent data too.

[pari_89]

Thank you, I have tried Newbler and it gives good results. By the way, any suggestions for reference-guided assembly tools??

I need to do a performance evaluation of reference against an assembly for mulitiple tools using Ion torrent data.

Thank you

[rboettcher]

Scripture and Cufflinks should be on your list for reference-guided / mapping assembly.