I am currently working with assembly programs and most of the assemblers output max contig length and N50. But many papers also report percent of reference genome covered by the contigs in their results. So if I have a set of contigs and a reference genome then how can I measure the percent of reference genome covered by the contigs of length greater than say x. I used NUCMER but I can get a delta or coords file as an output but not the exact percentage. Anyone knows of any tool which can output this parameter?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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