Hi all,
This is Illumina HiSeq2000 paired-end sequencing mapped to a reference transcriptome using Bowtie2 (using the default end-to-end alignment approach, if it matters). I took the mapped positions from the SAM file and generated a histogram, and the mode is easily base 1 on the scaffolds. Is there any particular reason for this? Is it something to be concerned about? Thanks for any feedback.
This is Illumina HiSeq2000 paired-end sequencing mapped to a reference transcriptome using Bowtie2 (using the default end-to-end alignment approach, if it matters). I took the mapped positions from the SAM file and generated a histogram, and the mode is easily base 1 on the scaffolds. Is there any particular reason for this? Is it something to be concerned about? Thanks for any feedback.
Comment