checking for bacterial distribution

Nullie

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Join Date: Jun 2013

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checking for bacterial distribution

06-03-2013, 01:02 PM

how can I check the bacteria distribution in a human gut?

If I extract a DNA sample and isolate all bacterial DNA from it can I then sequence all 16s rDNA's and somehow check the expression level of the different 16s' so it can inform me on the bacteria distribution? (like checking the expression level of some gene)
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Torst

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Join Date: Apr 2008

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06-06-2013, 12:13 AM

Yes you can!

Illumina-based analysis of microbial community diversity - PubMed

http://www.ncbi.nlm.nih.gov/pubmed/21677692

Microbes commonly exist in milieus of varying complexity and diversity. Although cultivation-based techniques have been unable to accurately capture the true diversity within microbial communities, these deficiencies have been overcome by applying molecular approaches that target the universally con …
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