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Does anyone have any experience or pointers for recycling IPAR servers into assembly workstations/server?
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Install Cent OS 5. Done.Originally posted by The_Roads View Post -
We put Fedora onto ours. We've got the Illumina pipeline and bowtie running really nicely. They're ideal machines to convert into mapping servers. You might want to up the memory a bit (I think ours had 16GB) for doing large assemblies, but other than that they're ready to go.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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