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  • newbietonextgen
    Member
    • Nov 2010
    • 56

    #1

    Freebayes 0.9.9 problems

    Hello All,

    I am trying to run freebayes version 0.9.9 and am having problems. I am used pervious versions and never encountered these problems.

    The command is use:

    freebayes -XuiP 0.01 -f /path_to_fasta_file -b 40077_sorted_RG.bam -b 40079_sorted_RG.bam -v trial.freebayes.vcf

    The output on the terminal: (never seen it doing this before)

    GACAGGCCAGGCAGAACAGACGGCTGCTTCATGTCGTCTGTGTCTACAGCAGCTATTGCAAAGGCCCATCCGTACCCCCTGGGATCCTTGAAATGCCCCTTCGGACGTAATCCGTACCGAGTATGCACGGAGCCCCTGGGCCCGTCACGACGGGATGCTTCTGCCGCTCAGTTCAGCGAGGCTCATTTTGGCCTCCAGCGCAGTCAGTCCTTGAGAGCCCCCGTGCGCACCACGAACGCAAAGCCAAACATAGCAAAGACAAACAGGAACAGTACTGCGGCACCTCCCATTGCCGCTCCTCGTACCGACTGAGTCATTATTGCGAGAACTGTGGGCAGCGTTCCCAGACGGCTTTCCGACTACAAATGAGTGCTTTCGGACCCGCTGTGCCCACTGCGGCGTGCCGCTGTTTCTCAGCTTTCCGCCACGACCCCTCCGTGAGGTCCTCGCTAAGAAGAGCGCTC
    1:2179
    Aborted

    The Vcf file shows the first SNP at 1:2179 and then it stops. The bam files (read group, index, started) were used with GATK and worked fine. The output is the same even of i use one bam file to call SNPs

    So what am i doing wrong?
    Thanks
    newbie..
  • newbietonextgen
    Member
    • Nov 2010
    • 56

    #2
    a little bit of digging around and i see that an error is also posted...

    Error: Exception: Cannot read past the end of the alignment's sequence.


    So what does this mean? Any ideas?

    Comment

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