Hi everyone,
after successfully using MG-RAST with assembled data, I am trying to use it with raw fastq illumina sequences. I originally had two fastq files (forward and reverse paired ends); I merged them and uploaded the file.
I now receive an error message:
Warning: The unique id count does not match the sequence count. You will not be able to use this file for submission.
Basically the unique id count is half the number of sequences.
My reads are ordered as forward and reverse with the following format:
@HWUSI-EAS1700R:25:FC:6:1:12466:1106 1:Y:0:TTAGGC
and
@HWUSI-EAS1700R:25:FC:6:1:12466:1106 2:Y:0:TTAGGC
My guess is that I may need to modify the header. Any suggestion?
Thanks
Max
- Edit: I should be able to modify the header by myself (I know a little bit of Python), but I am not sure if that is the problem and what my header should be.
Thanks again
Max
after successfully using MG-RAST with assembled data, I am trying to use it with raw fastq illumina sequences. I originally had two fastq files (forward and reverse paired ends); I merged them and uploaded the file.
I now receive an error message:
Warning: The unique id count does not match the sequence count. You will not be able to use this file for submission.
Basically the unique id count is half the number of sequences.
My reads are ordered as forward and reverse with the following format:
@HWUSI-EAS1700R:25:FC:6:1:12466:1106 1:Y:0:TTAGGC
and
@HWUSI-EAS1700R:25:FC:6:1:12466:1106 2:Y:0:TTAGGC
My guess is that I may need to modify the header. Any suggestion?
Thanks
Max
- Edit: I should be able to modify the header by myself (I know a little bit of Python), but I am not sure if that is the problem and what my header should be.
Thanks again
Max
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