Blast error when choosing m8

[maimaiti2008]

When I was running blastall -p blastn and choosing m8 as the output format, I was told as follows

[blastall] ERROR: ncbiapi [000.000] SEQTITLE: BioseqFindFunc: couldn't uncache

If I changed the output format to m1, the error message disappeared.

Is there anyone who can tell me how to deal with it?

Thanks!

[GenoMax]

Are you using an older version of blast package? Did you create the blast index files yourself?

[maimaiti2008]

Are you using an older version of blast package? Did you create the blast index files yourself?

Yes, I used the newest one 2.2.9 and made the database myself

[GenoMax]

Are you running the older "legacy_blast.pl" script or using the new blastn -task blastn command line. If it is the latter then should you not be using -outfmt '6'?

Can you post your actual command line?

[maimaiti2008]

Thanks! I havbe solved the problem. I downloaded the most recent version 2.2.26 and successfully run it.

[maimaiti2008]

Thanks!

Are you using an older version of blast package? Did you create the blast index files yourself?

Thanks! I havbe solved the problem. I downloaded the most recent version 2.2.26 and successfully run it.